Table 1.

Yeast two-hybrid analysis of the interaction between TbTim54 and other TbTims.

Interacting Partner	Growth at 0 mM AT	Growth at 2.0 mM AT	Growth at 3.5 mM AT	Growth at 5.0 mM AT
TbTim54AD + TbTim9 BD	−	−	−	−
TbTim54AD + TbTim10 BD	+	−	−	−
TbTim54AD + TbTim8/13 BD	++	−	−	−
TbTim54AD + TbTim11 BD	++	++	++	++
TbTim54AD + TbTim12 BD	+	−	−	−
TbTim54AD + TbTim13 BD	++	+	+	−
TbTim54AD + TbTim54 BD	−	−	−	−
TbTim54AD + TbTim17-N(1–30)	++	+	−	−
TbTim54AD + △30-TbTim17	−	−	−	−
pGADT7 + pGBKT7	−	−	−	−
SV40-T-AD + P53-BD	++	++	++	++

Growth of yeast cells transformed with the respective pairs of AD and BD plasmids on SD medium lacking leucine, tryptophan, and histidine (−leu/−trp/−his) supplemented with various concentrations (up to 5 mM) of AT is indicated by ‘+’. ‘++’ indicates heavier growth and ‘−’ indicates no growth.