Fig. 5.

CD4⁺ and CD8⁺ T cells from LCK^CRE Bim^fl/fl mice were differentially sensitive to apoptotic stimuli compared to age-matched WT controls. a CD4⁺, b CD8⁺ splenic T cells, and c CD4⁺CD8⁺ thymocytes from young mice were cultured for 24 and 48 h in the absence of cytokines, 1 µg/mL ionomycin, 4 ng/mL PMA, or 1 µM etoposide. Viability was assessed by Annexin V/PI staining with Annexin V^neg PI^neg cells considered viable. n ≥ 4 for each age group and genotype. Data represented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.