Fig. 6.

CD4⁺ and CD8⁺ T cells from LCK^CRE Bim^fl/fl mice upregulate expression of other BH3-only proteins in response to loss of BIM. mRNA expression levels of BCL-2 proteins from CD4⁺ and CD8⁺ splenic T cells from a young and b old LCK^CRE Bim^fl/fl mice. Age-matched WT mice were used as a reference sample. Expression was normalized using B2M and analyzed using the ∆∆C_T method. n ≥ 3 for young and n ≥ 4 for old age group and genotype. Significance was compared to age-matched reference samples (normalized to 1, dashed line) Data represented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by calculating RQ Min/Max with a 95% confidence level using the ExpressionSuite Software