Fig. 3. Zuranolone exhibits synergistic activity with the benzodiazepine diazepam.

A. Concentration-response curves of zuranolone, DZP, and a combination at α1β2γ2-expressing Ltk cells. Percent potentiation is plotted relative to the amplitude of GABA (2 μM) alone. The horizontal dotted line corresponds to the effect level plotted in the isobologram. B. Isobologram of the 150% effect level. The concentration of DZP applied alone required to reach this effect was 360 nM, the concentration of zuranolone applied alone was 315 nM. When coapplied with 100 nM DZP, only 10 nM of zuranolone was required potentiate the current greater than 150%. C. Concentration-response curves of zuranolone, DZP, and a combination at α₄β₃δ-expressing cells. Percent potentiation is plotted relative to the maximum GABA response.