TABLE 1.

Bacterial taxa used in the in vitro model^d

Bacterial strain or species	Phylum	Oxygen status	Gram status	Isolation (source)	Health implications	Genus frequency across gut microbiota (%)a	Multidrug export genes	Moco biosynthesis gene(s)b
C. innocuum ATCC 14501	Firmicutes	Anaerobe	Positive	Appendiceal abscess (Lurie Children's Hospital of Chicago)	Commensal; model for sporulation	89.5	mdtK, mepA, norM	moaA
L. rhamnosus	Firmicutes	Facultative	Positive	Vaginal swab (Washington University, St. Louis)	Commensal; probiotic	14.5	emrB, mdtD mepA, yheH, yheI
E. faeciumc	Firmicutes	Facultative	Positive	Bloodstream (Washington University, St. Louis)	Opportunistic pathogen	4.3	emrB, yfmO, yheH, yheI
E. coli K-12 MG1655	Proteobacteria	Facultative	Negative	Stool (Palo Alto, CA)	Commensal	75.7	acrD, acrE, acrF, bcr, emrA, emrB, emrD, emrE, emrK, emrY, mdfA, mdlA, mdlB, mdtA, mdtB, mdtC, mdtD, mdtE, mdtF, mdtG, mdtK, mdtL, mdtM, sdsP, sdsQ, sdsR, yojI	moaA, moaB, moaC, moaD, moaE, modA, modB, modC, moeA, moeB, mog
K. pneumoniae	Proteobacteria	Facultative	Negative	Bloodstream (Washington University, St. Louis)	Opportunistic pathogen	9.8	acrA, acrB, acrE, acrF, acrZ, emrA, emrB, emrD, mexA, mexB, mdfA, mdlB, mdtA, mdtB, mdtC, mdtD, mdtH, mdtK, mdtL, mdtM, mdtN, mdtO, oqxB19, stp, ybhF, ybhR, ybhS, yheI	moaA, moaB, moaC, moaD, moaE, moaF, modA, modB, moeA, moeB, mog

^aFrequency (%) computed from the gut-associated metagenome samples in the Human Microbiome Project II (48) (n = 553).

^bGenes associated with molybdenum cofactor biosynthesis (22), including those described as critical for anaerobic deglycosylation of doxorubicin (13).

^cSpecies confirmed from processing16S rRNA gene amplicon sequences in BLAST. Multidrug export and Moco biosynthesis genes were screened from the human microbiome project reference genome for E. faecium (GenBank Assembly accession no. GCA_000174395.2).

^dGenes reported were based on whole-genome sequence assemblies for the isolates or, where indicated, reference genome.