FIG 2.
Coexpression of briC with the fab gene cluster promotes biofilm development. (A and C) Representative confocal microscopy images showing top view of the reconstructed biofilm stacks of term− and term+ cells in strain R6D stained with SYTO59 dye at 72 h in (A) a WT and (C) a ΔcomE//briC-OE genomic background. Images are pseudocolored according to depth (scales shown). (B and D) COMSTAT2 quantification of 72 h biofilm images. y axis denotes units of measurement: μm3/μm2 for biomass and μm for maximum thickness and average thickness over biomass. Error bars represent standard error of the mean calculated for biological replicates (n = 3); **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001 using Student’s t test.