FIG 4.

BriC promotes a membrane composition enriched in unsaturated fatty acids. Mass spectrometry analysis of the PG molecular species of R6D pneumococcal strain and its isogenic mutants. Representative spectrum of PG molecular species of (A) WT, (B) ΔbriC, and (C) briC-OE strains. The most unsaturated molecular species for each carbon number is highlighted in red. (D) The PG species from three biological replicates were quantified and the ratios for each carbon number were calculated. P values were calculated in Prism software using ANOVA followed by Tukey’s test for multiple comparisons.