FIG 3.
Deletion mutant assays and medium influent assays to explore the causes of mutual enhancement between P. aeruginosa and C. albicans in biofilms. (A) Biovolume of C. albicans grown in dual-species biofilms with P. aeruginosa deletion mutants at 24 h (see main text for mutant descriptions, n = 9 to 24). (B) Biovolumes of mono- and dual-species P. aeruginosa biofilms at 24 h (n = 9 to 24). All error bars indicated are standard errors. (C) The mean values of C. albicans biovolume are plotted against the corresponding mean value of wild-type (WT) P. aeruginosa PA14 and mutant biovolume from their respective dual-species biofilms. There is no significant correlation between the two (linear correlation analysis; P = 0.391; r2 = 0.107). *, P < 0.05 by Wilcoxon signed-rank test with Bonferroni correction.