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. 2021 Jun 23;6(3):e00416-21. doi: 10.1128/mSphere.00416-21

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Copyright © 2021 Kasetty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Biomass accumulation, density correlation analysis, and visualization of C. albicans in coculture with different CF clinical isolates of P. aeruginosa. (A) Biovolume of C. albicans grown as dual-species biofilms with P. aeruginosa clinical isolates along with wild-type (WT) PA14 for comparison at 24 h (n = 18). (B) Biovolumes of P. aeruginosa clinical isolates in monococulture and dual culture with C. albicans at 24 h (n = 18). (C) Global density correlation measurements of WT P. aeruginosa and clinical isolates and C. albicans biofilms (n = 6). *, P < 0.05. (D) Visualization of dual-species biofilms of P. aeruginosa and C. albicans. From top to bottom, spatially resolved density correlation, 3-D renderings of dual-species biofilms, C. albicans channel split, and P. aeruginosa channel split.