TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Porphyromonas gingivalis | ||
| ATCC 33277 | Wild type | |
| YS19181 | ΔporX::EmR | (7) |
| YS17717 | ΔsigP::EmR | This work |
| W83 | Wild type | |
| YS19145 | ΔporX::EmR | (7) |
| YS18145 | ΔsigP::EmR | This work |
| E. coli | ||
| DH5α | F− supE44 ΔlacU169 (ϕ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection |
| (21) | ||
| BL21(DE3) | F− ompT hsdSB (rB− mB− ) gal dcm (DE3) | (22) |
| Plasmids | ||
| pT-COW | repColE1 AmpR Tcr | (14) |
| pYS18679 | repColE1 reppB8-51 AmpR CamR TcR PPGN_1016 porXCDS | (7) |
| pYS19107 | repColE1 reppB8-51 AmpR CamR TcR | This work |
| sigPCDS | This work | |
| pET28a | repColE1 KmR lacI PT7 | Novagen |
| pET11a | repColE1 AmpR lacI PT7 | Novagen |
| pET21a | repColE1 AmpR lacI PT7 | Novagen |
| pGEM-T-Easy | reppMB1, f1 AmpR lacZ-α | Promega |
| pGEM-ermF | repColE1 AmpR ermF lacI PT7 | (16) |
| pYS17676 | repColE1 AmpR ermF lacI PT7 sigP8-305nt | This work |
| pYS18456 | repColE1 AmpR lacI PT7 porX (PGN_1019) | (7) |
| pYS18051 | repColE1 KmR lacI PT7 rpoA (PGN_1841) | This work |
| pYS18943 | repColE1 AmpR lacI PT7 rpoB (PGN_1571) | This work |
| pYS18165 | repColE1 KmR lacI PT7 rpoC (PGN_1570) | This work |
| pYS18052 | repColE1 KmR lacI PT7 rpoD (PGN_0638) | This work |
| pYS18056 | repColE1 KmR lacI PT7 sigP (PGN_0274) | This work |