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The Kp qPCR assay accurately quantifies K. pneumoniae, K. variicola, and K. quasipneumoniae. Eleven Klebsiella clinical isolates were extracted and amplified by the Kp qPCR assay, each with 3 technical replicates. (A) Quantification of each isolate relative to KPPR1 set as 100%. (B) Alignment of the amplicons of the 11 isolates with the fiu (ybiL) primers and probe.
