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LAMP-OSD analysis of SARS-CoV-2 genomic RNA. The indicated numbers of copies of SARS-CoV-2 genomic RNA per reaction were analyzed using NB, Tholoth, and both 5-primer and 6-primer Lamb LAMP-OSD assays. Negative-control assays received 23 ng of human genomic DNA. Images of endpoint OSD fluorescence taken after 90 min of amplification are shown. Data are representative of four biological replicates.
