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Multiplex LAMP-OSD analysis of SARS-CoV-2 genomic RNA. The indicated numbers of copies of SARS-CoV-2 genomic RNA per reaction were amplified at 65°C using NB plus Tholoth (NB + Tholoth) (A) or NB + 6-Lamb (B) multiplex LAMP-OSD assays for 90 min and 60 min, respectively. The control reaction received 23 ng of human genomic DNA. Images of OSD fluorescence captured after completion of amplification are shown. Data are representative of three biological replicates.
