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. 2021 May 19;6(3):e00911-20. doi: 10.1128/mSphere.00911-20

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Copyright © 2021 Bhadra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — LAMP-OSD analysis of SARS-CoV-2 virions in the presence of human saliva. The indicated numbers of copies of virions per reaction were analyzed by individual or multiplex (Mx) LAMP-OSD assays in the presence of TE buffer or human saliva. Endpoint images of OSD fluorescence taken after 90 min of amplification are shown for Tholoth (A), 6-Lamb (B), and NB (C) individual LAMP-OSD assays and NB + Tholoth multiplex LAMP-OSD assays (D). Endpoint images of OSD fluorescence taken after 60 min of amplification are shown for NB + 6-Lamb multiplex assays (E and F). BL, blank tubes lacking any reaction mixtures or templates. Data are representative of four biological replicates.