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. Author manuscript; available in PMC: 2022 Jun 23.
Published in final edited form as: J Am Chem Soc. 2021 Jun 10;143(24):9191–9205. doi: 10.1021/jacs.1c04155

Figure 4.

Figure 4.

Inhibition of the BFC activity of recombinant CYP3A4 by 4, 7, and the control complex [Ru(tpy)(Me2bpy)(Cl)]Cl. Inhibitory assays were conducted at room temperature in a reconstituted system containing 0.2 μM CYP3A4 and 0.3 μM cytochrome P450 reductase by monitoring formation of a fluorescent product. The remaining activity was calculated relative to the DMSO-containing sample, used as a control (100% activity). The IC50 values were derived from fittings to the [% activity] vs [inhibitor] plots.