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. 2021 Jun 18;7(7):1191–1204. doi: 10.1021/acscentsci.1c00361

Figure 1.

Figure 1

CART delivery platform methodology effectively complexes, delivers, and releases mRNA via both systemic and local administration. (A) CART electrostatic formulation, cellular uptake, endosomal escape, and translation of SARS-CoV-2 RBD mRNA. (B) CART synthesis via ring-opening polymerization (DBU = 1,8-diazabicyclo[5.4.0]undec-7-ene, TU = 1-(3,5-bis(trifluoromethyl)phenyl)-3-cyclohexylthiourea). (C) CART chemical structure, degradation products, and charge-altering mechanism. (D) In vivo luciferase reporter gene expression via systemic IV administration (left, 5 μg of fLuc mRNA), and local IM administration (2.5 μg of fLuc mRNA each flank). (E) Quantification of in vivo mRNA expression at 4 h postadministration.