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. 2021 Jun 18;7(7):1191–1204. doi: 10.1021/acscentsci.1c00361

Figure 2.

Figure 2

Addition of CpG to RBD mRNA-CART elicits a stronger anti-RBD immunoglobulin response and leads to earlier isotype switching. (A) BALB/c mice were immunized intravenously with either 3 μg of RBD mRNA-CART (n = 5), 3 μg of RBD mRNA-CART plus 3 μg of CpG (n = 5), 3 μg of CpG CART (n = 5), or Naïve untreated (n = 5) and boosted on day 4 and day 8 after priming. (B) Serum levels of RBD-specific IgGs from RBD mRNA-CART (black), RBD mRNA + CpG-CART (blue), CpG CART (orange), and Naïve (red) mice were monitored over the course of 60 days postpriming by ELISA. (C) On day 14 and day 60 after priming, the distribution of IgG isotypes specific to RBD was analyzed using antimouse IgG1 (blue), IgG2a (black), IgG2b (red), and IgG3 (gray) monoclonal antibodies by ELISA. (D) On day 14 and day 60 after priming, the absolute concentration of the anti-RBD IgG was evaluated. * = P < 0.05 unpaired Student’s t test (two-tailed). Data representative of 2 individual experiments.