Skip to main content
. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Biopolymers. 2021 Apr 1;112(6):e23427. doi: 10.1002/bip.23427

Figure 1.

Figure 1.

A) Ionic Exchange Chromatography (IEC) chromatogram of the complex of oxy (HbII-III) using the HiPrep 16/10 column. The components present at 45 seconds and 75 second elution times are HbII and HbIII, respectively. B) Ionic Exchange Chromatography (IEC) of Oxy(HbII-III) complex with a new column, XK 16/70 packed with Q Sepharose high performance (Q HP) matrix with a column pH of 7.5. The first peak represents the OxyHbII (150 elution time) component, and the second peak is OxyHbIII (450 elution time). C). SDS-PAGE profile for HbII and HbIII separation: lane 1 empty, lane 2 and 6 are molecular-weight markers, lane 3 HbII, lane 4 HbII-III, and lane 5 HbIII.