FIGURE 2.

CT‐001 and CT‐001(Sial) demonstrate enhanced binding to human platelets activated with human thrombin and convulxin. Activated platelets from three healthy independent donors were washed and exposed to FVIIa variants, and the binding to cell surfaces was detected by Alexa488‐anti‐FVII (clone AA3) using flow cytometry. The mean FL1 of platelet population was measured and expressed as a percent of maximum FL1 achieved by each study (n=3 independent healthy donors). CT‐001 showed significantly increased binding to activated platelets in comparison to dWT FVIIa and WT FVIIa. ANOVA, analysis of variance; dWT, desialylated wild‐type; FVIIa, activated factor VII