FIGURE 6.

Desialylated FVIIa variants have increased clearance by hepatocytes in vitro. CT‐001, which has four desialylated N‐glycans, was cleared by hepatocytes from supernatant significantly faster than CT‐001(Sial) and WT FVIIa. dWT FVII, which has two desialylated N‐glycans, was removed at a slower rate than CT‐001. The majority of CT‐001(Sial) and WT FVIIa was recovered in the supernatant after 60 minutes of incubation with hepatocytes. Three independent studies were performed. dWT, desialylated wild‐type; FVII, factor VII; FVIIa, activated factor VII; WT, wild‐type