Figure 3.
Shox2 IN intrinsic and firing properties from uninjured and SCI mice. A–D, Comparisons of intrinsic properties of Shox2 INs from uninjured (black bars) and SCI (red bars). There were no significant differences in (A) membrane potential (Em), (B) input resistance (Rin), or (C) rheobase current. D, Action potential threshold was significantly more depolarized in Shox2 INs from SCI mice (unpaired t test, p = 0.03). Dots represent individual data points. Bar graphs represent mean ± SD. E, Examples of Shox2 IN responses to a 50 pA depolarizing current from uninjured (black trace) and SCI (red trace) mice. F, Mean firing frequency/current response and linear fit for uninjured (black) and SCI (red). G, Pie charts represent incidence of Shox2 IN firing types from uninjured (top) and SCI (bottom) mice. Gray represents tonic firing. Teal represents initial burst firing. Purple represents delay firing. H, Examples of Shox2 INs from uninjured (black) and SCI (red) mice spontaneously oscillating in control recoding solution.