Figure 2.

Effects of hsa_circ_0044235 on CIA mouse model. (a) Induction of CIA and hsa_circ_0044235 injection in DBA/1 J mice immunized with CII. (b) Clinical case scores of mice in CIA, CIA+Ad-Vec (adenovirus-vector), CIA+Ad-hsa_circ_0044235 groups. (c) Representative left knee joints of mice in each group on day 32 after the first CII immunization. (d) Hematoxylin and eosin (H&E) staining was performed to detect knee joint injuries in each group of mice. (e) The TUNEL method was constructed to measure the number of apoptotic cells in synovial tissues. (f-h) The levels of IL-1β, IL-6 and TNF-α in the synovial tissues of the knee joint were detected by ELISA. (i) The expression of hsa_circ_0044235 in the knee joint synovial tissues of each group of mice was detected by RT-qPCR. CIA: collagen-induced arthritis; CII: bovine type II collagen. ELISA: enzyme-linked immunosorbent assay; IL: interleukin; TNF: Tumor necrosis factor. ***P < 0.001 vs. CIA+Ad-Vector