Figure 4.

The effect of hsa_circ_0044235 overexpression and SIRT1 silencing on RA-FLSs cells. After transfection with hsa_circ_0044235 overexpression or siSIRT1 or the corresponding blank vector, the cells were incubated with LPS/ATP as needed (synovial cells were first treated with 1 μg/ml LPS for 6 h, and then incubated with 3 mM ATP for 1 h). (a) RT-qPCR was used to detect the expression of hsa_circ_0044235 in each group of cells. (b-d) the expression of SIRT1 in each group of cells was determined by RT-qPCR and Western blot. (e-f) The number of caspase-1 positive cells in each group was evaluated by flow cytometry. (g) The content of caspase-1 in cells of each group was detected by immunofluorescence. experiments were repeated in triplicates. ***P < 0.001 vs. control; ^###P < 0.001 vs. LPS/ATP+NC; &&&P < 0.001 vs. LPS/ATP+siNC; +++P < 0.001 vs. LPS/ATP+circ_0044235; ^{^^^}P < 0.001 vs. LPS/ATP+siNC+circ_0044235