Figure 3.

Function of hsa_circ_0006168 as miR-628-5p sponge in human GBM cells. (a) The predicted binding sites of miR-628-5p in hsa_circ_0006168 wt were mutated. (b) Dual-luciferase reporter assay measured luciferase activity of hsa_circ_0006168 wt and mut in 293 T cells transfected with miR-628-5p mimic or mimic NC. (c) RT-qPCR detected the enrichment of hsa_circ_0006168 in cell lysate incubated with Bio-miR-628-5p or Bio-NC in A172 and LN229 cells. (d, e) RT-qPCR detected miR-628-5p expression in Normal brain and GBM tissues, as well as cells including A172, LN229 and HEB. (f) Pearson correlation coefficient analysis validated the linear correlation between hsa_circ_0006168 and miR-628-5p expression in GBM tissues. *P < 0.05