Figure 4.

Effect of miR-628-5p downregulation on role of hsa_circ_0006168 deficiency in cell proliferation and motility of human GBM cells in vitro. A172 and LN229 cells without and with hsa_circ_0006168 knockdown were transfected with miR-628-5p inhibitor or inhibitor NC for 48 h. (a, b) RT-qPCR detected miR-628-5p expression. (c) CCK-8 assay monitored OD value at 450 nm after inoculation of transfected cells for 0–72 h. (d, e) Transwell assays determined numbers of migrated cells and invaded cells after inoculation of transfected cells for 48 h. (f) Western blotting measured relative protein expression of E-cadherin, Vimentin and Snail. *P < 0.05