Figure 6.

Effects of miR-628-5p and IGF1R overexpression in cell proliferation and motility of human GBM cells in vitro. (a) RT-qPCR detected miR-628-5p expression in A172 and LN229 cells transfected with miR-628-5p mimic or mimic NC. (b-i) A172 and LN229 cells without and with miR-628-5p overexpression were co-transfected with pcDNA-IGF1R vector or pcDNA empty vector for 48 h. (b, c) Western blotting detected relative IGF1R protein expression. (d) CCK-8 assay monitored OD value at 450 nm after inoculation of transfected cells for 0–72 h. (e, f) Transwell assays determined numbers of migrated cells and invaded cells after inoculation of transfected cells for 48 h. (g) Western blotting measured relative protein expression of E-cadherin, Vimentin and Snail. *P < 0.05