Fig.3. Effects of sugar chain decomposition enzymes or cross-linkers on the bands of ApoM on the western blots .

(A, B) Glycated HDL was incubated with the sugar chain decomposition enzymes, PNGase F or glycosidase (G) and/or neuraminidase (N), followed by western blot analysis for ApoM. (C, D) HDL was incubated with vehicle (C) or succinimidyl 4,4’-azipentanoate (LC-SDA, S), disuccinimidyl suberate (DSS, D), or bismaleimidoethane (BMOE, B), followed by western blot analysis for ApoM.