Fig.4. Modulation of the S1P-binding capacity of glycated HDL .

(A) Modulation of the contents of S1P in HDL treated or not treated with methylglyoxal (MG) ( n =4). (B) Modulation of the S1P levels in standard solutions treated or not treated with 6 mM of MG ( n =3). (C) The binding capacities of HDL treated with various concentrations of MG for 24 hours were evaluated by the pattern of redistribution of C ₁₇ S1P between HDL and albumin ( n =3–4). (D) Time-course of C ₁₇ S1P levels in HDL treated or not treated with 6 mM of MG incubated on HEK293 cells ( n =4). The results are presented as the ratio of C ₁₇ S1P levels on HDL treated and not treated with MG to each C17S1P level at 0.5 hour. ^＊ P ＜0.01, ^＊＊ P ＜0.05.