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. 2021 May 11;27(8):941–950. doi: 10.1111/cns.13656

FIGURE 3.

FIGURE 3

DRN 5‐HT neurons facilitated the emergence from isoflurane anesthesia. A, Top, schematic illustration of virus injection and verification of virus expression (red, hM3Dq/hM4Di‐mCherry; green: Tph2). Bottom, experimental protocol of assessment of time to LORR and RORR. B, C, Confirmation of hM3Dq or hM4Di virus efficiency. D, Activation of DRN 5‐HT neurons did not change the induction time (P = 0.6460). E, Activation of DRN 5‐HT neurons significantly shortened the emergence time (P = 0.0217). F, Inhibition of DRN 5‐HT neurons did not change the induction time (P = 0.4252). G, Inhibition of DRN 5‐HT neurons significantly prolonged the emergence time (P = 0.0135) (*P < 0.05, **P < 0.01, and ***P < 0.001, two‐way ANOVA followed by Sidak's post hoc test)