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. 2021 Jul 8;17(7):e1009672. doi: 10.1371/journal.ppat.1009672

Fig 2. Strategies for the development of CRISPR-based antimicrobials.

Fig 2

(A) One approach is to deliver a complete exogenous CRISPR/Cas system to target a desired locus (chromosome or plasmid). Alternatively, if the target pathogen harbors a CRISPR/Cas system, one can deliver a CRISPR array and exploit the native Cas proteins to drive DNA targeting. (B) CRISPR antimicrobials can be designed to target a chromosomal locus, leading to cell death or a plasmid locus, leading to plasmid loss and sensitization of the target bacteria. Left: an array of different CRISPR antimicrobial delivery vehicles proposed by different research groups. Created with BioRender.com. Cascade, CRISPR-associated complex for antiviral defense; CRISPR/Cas, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein; SAPI, staphylococcal pathogenicity island.