Fig 4. Previous DENV-Immunity induced by vaccination does not enhance ZIKV disease in mice.
Mice were immunized intramuscularly (i.m.) on weeks 0 with 1 μg of each DENV serotype of the purified inactivated vaccine (DENV-PIV) or 102 PFU of the live attenuated tetravalent DENV vaccine (TDENV-LAV) and vaccinated on weeks 4 and 8 with 50 μg of the DNA-M.ENV vaccine and 1 μg of the purified inactivated ZIKV (ZPIV). Mice received a single immunization with 109 vp of the RhAd52-M.ENV vaccine. Mice were challenged i.v. with 102 PFU ZIKV-BR 4 weeks after DNA-M.ENV and ZPIV vaccination and 8 weeks after RhAd52-M.ENV vaccination. (A) ZIKV mRNA viral loads in serum. Data is representative of one experiment with 5–10 animals per group. Each line represents an individual mouse.