Table 3. In silico analysis of VUS identified in BRCA1 and BRCA2 using mutation function prediction models.

Gene	HDVS cDNA	HGVS protein	SIFT	PolyPhen	Align-GVGD	Provean	Human Splicing Finder	ID
BRCA1	c.3305A>G	p.Asn1102Ser	Tolerated	Benign	Class C0	Deleterious	Creation of an exonic ESS site. Potential alteration of splicing.	49
	c.3752G>A	p.Cys1251Tyr	Tolerated	Benign	Class C0	Neutral	Alteration of an exonic ESE site. Potential alteration of splicing.	48
BRCA2	c.3371A>G	p.Gln1124Arg	Damaging	Probably Damaging	Class C35	Deleterious	Activation of an exonic cryptic donor site. Potential alteration of splicing.	24
	c.8942A>G	p.Glu2981Gly	Tolerated	Benign	Class C65	Neutral	ND	12