FIGURE 2.

In vitro nuclease activity and biofilm-forming ability of ST1792 and its isogenic mutant strains. (A) Toluidine blue DNA agar test with red zones representing nuclease activity. (B–D) Biofilm-forming ability of tested strains on polypropylene 96-well plates (B, WT = 1.135 ± 0.055), titanium disk (C, WT = 1.237 ± 0.091), and UHMWPE disk (D, WT = 1.457 ± 0.119). Biofilm biomass was stained with crystal violet. Statistical significance was calculated using ANOVA with Dunnett multiple column comparisons. n = 3/group for each experiment. *p < 0.05; **p < 0.01; ***p < 0.001 vs. WT.