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Role of nuc1 and nuc2 on bacterial aggregation and biofilm structure in vitro. (A) Biofilm formed by ST1792 and its isogenic mutant on titanium disk observed with SEM (scale bar = 1 mm). (B) Flow cytometry was used to determine bacterial aggregation. (C) Quantification result for the flow cytometry experiment. Statistical significance was calculated using ANOVA with Dunnett multiple column comparisons. n = 3/group. **p < 0.01 vs. WT.
