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. 2021 Jul 3;8(4):ENEURO.0066-21.2021. doi: 10.1523/ENEURO.0066-21.2021

Figure 8.

Figure 8.

Cdh11−/− neurons show altered calcium activity. A, Example calcium current traces from Cdh11 WT and KO hippocampal cultures at 15 DIV recorded for 3 min using NeuroBurst Orange lentivirus calcium indicator. Averaged fluorescence intensity per cell of 1940 active neurons (WT) and 1009 active neurons (KO) was plotted against time (seconds). B, Total cell count of Cdh11 WT and KO cultures using DAPI counterstain at 16 DIV. N = 6 WT and 7 KO cultures. C, Infection rate of Cdh11 WT and KO neurons at 16 DIV infected by the IncuCyte Neurolight Orange lentivirus. The number of infected cells was normalized to the total number of neurons counterstained with DAPI. N = 8 WT and 7 KO cultures. Measurement of (D) number of active neurons, (E) correlation of activity, (F) mean burst strength, and (G) mean burst rate of Cdh11 WT and KO hippocampal neurons infected with the NeuroBurst Orange lentivirus calcium indicator recorded at 15 DIV and 16 DIV; *p < 0.05, **p < 0.01, ***p < 0.001, unpaired two-tailed t test. N = 6 WT and 7 KO cultures from two different time points.