Table 2.
Secondary antibodies
| Secondary antibodies | ||||||
|---|---|---|---|---|---|---|
| Antibody | Host, isotype | Immunogen | Source | Catalog number | Clone no./RRID | Dilution |
| Anti-rabbit IgG, HRP conjugate | Goat, IgG | IgG rabbit | Cell Signaling Technology | 7074P2 | RRID:AB_2099233 | 1:7500 |
| Anti-mouse IgG, HRP conjugate | Goat, IgG | IgG mouse | Thermo Fisher Scientific | A16072 | RRID:AB_2534745 | 1:5000 |
| Anti-goat IgG, HRP conjugate | Donkey, IgG | IgG goat | Thermo Fisher Scientific | A15999 | RRID:AB_2534673 | 1:5000 |
| Anti-mouse Alexa Fluor 488 | Donkey, IgG | IgG (H + L) mouse | Thermo Fisher Scientific | A21202 | RRID:AB_141607 | 1:1000 |
| Anti-mouse Alexa Fluor 647 | Donkey, IgG | IgG (H + L) mouse | Thermo Fisher Scientific | A31571 | RRID:AB_162542 | 1:1000 |
| Anti-rabbit Alexa Fluor 488 | Donkey, IgG | IgG (H + L) rabbit | Thermo Fisher Scientific | A21206 | RRID:AB_2535792 | 1:1000 |
| Anti-rabbit Alexa Fluor 568 | Donkey, IgG | IgG (H + L) rabbit | Thermo Fisher Scientific | A10042 | RRID:AB_2534017 | 1:1000 |
| Anti-rabbit Alexa Fluor 647 | Donkey, IgG | IgG (H + L) rabbit | Thermo Fisher Scientific | A31573 | RRID:AB_2536183 | 1:1000 |
| Anti-guinea pig Alexa Fluor 488 | Donkey, IgG | IgG (H + L) guinea pig | Jackson ImmunoResearch | 706545148 | RRID:AB_2340472 | 1:1000 |
| Anti-guinea pig Alexa Fluor 568 | Goat, IgG | IgG (H + L) guinea pig | Thermo Fisher Scientific | A11075 | RRID:AB_2534119 | 1:1000 |
List of secondary antibodies used for Western blotting and immunocytochemistry. HRP, horseradish peroxidase.