Table 1.

In vitro antiproliferative activities (IC₅₀, µM)^a,b of the synthesised compounds 2–12.

Compound	In vitro cytotoxicity (µM)					WI38c
	HePG2	HCT-116	MCF-7	PC3	HeLa
2	16.08 ± 1.4	8.81 ± 0.7	8.36 ± 0.9	26.82 ± 2.2	39.03 ± 2.4	86.48 ± 4.7
3	40.02 ± 2.5	10.84 ± 1.0	27.36 ± 1.8	44.50 ± 3.5	68.98 ± 3.9	63.1 ± 3.43
4	58.75 ± 3.3	72.40 ± 3.8	55.04 ± 3.2	14.27 ± 1.3	21.10 ± 1.6	nt
5	83.56 ± 4.4	94.14 ± 5.1	>100	94.60 ± 4.9	75.19 ± 4.8	nt
6	79.04 ± 4.2	56.36 ± 3.2	81.58 ± 4.0	>100	91.90 ± 5.1	204 ± 11.1
7	53.43 ± 2.9	38.63 ± 2.0	40.83 ± 2.5	70.10 ± 3.8	73.29 ± 4.2	59.49 ± 3.23
8	23.67 ± 1.8	13.85 ± 1.3	17.28 ± 1.4	36.69 ± 2.8	58.76 ± 3.7	151.4 ± 8.22
9	44.70 ± 2.7	29.52 ± 1.8	35.17 ± 2.2	51.06 ± 3.6	73.59 ± 4.1	nt
10	34.32 ± 2.1	27.49 ± 1.5	19.69 ± 1.6	30.90 ± 2.6	46.17 ± 3.0	nt
11	20.43 ± 1.6	46.13 ± 2.7	14.55 ± 1.3	18.75 ± 1.5	32.15 ± 1.9	94.49 ± 5.13
12	70.46 ± 3.8	49.72 ± 2.9	61.53 ± 3.5	93.48 ± 4.4	85.44 ± 4.7	76.26 ± 4.14
DOX	4.50 ± 0.2	5.23 ± 0.3	4.17 ± 0.2	8.87 ± 0.6	5.57 ± 0.4	55.29 ± 3.0
Afatinib	5.5 ± 0.24	11.2 ± 1.11	7.2 ± 0.50	7.6 ± 0.60	6.3 ± 0.69	nt

^aIC₅₀ values for each cell line are the compound concentration that inhibits 50% of the cell growth measured by MTT assay. ^bEach value was reproduced in triplicate. ^cNon tumour normal cell.