Figure 6.

The effect of tetralone derivatives on NF-κB production. NF-κB activation in macrophages was evaluated by using RAW-Blue™ cells. Macrophages were pre-treated with tetralones for 30 min and they were induced by 1 μg/ml LPS for 24 h. The activity of secreted embryonic alkaline phosphatase was measured by using QUANTI-Blue™ detection medium. The optical density was measured at 600 nm. Data are presented as means ± SD in percentage of LPS-treated group; n = 3 × 6 (pooled data of three independent experiments with six parallels); Student’s t test, p values <0.05 were considered significant. ***p < 0.001; n.s.=non-significant.