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. 2021 Jun 22;13(12):16367–16380. doi: 10.18632/aging.203162

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Copyright: © 2021 Liao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Characterization of patient-derived S100A9⁺ cells. Multiple immunofluorescence staining shows DAPI (gray), S100A9 (green), CD34 (blue, A), CD56 (pink, D), CD20 (purple, G), and CD3 (orange, J) expression and coexpression (double-positive cells) in HCC tissue. Quantification of CD34 (B), CD56 (E), CD20 (H), and CD3 (K) cell densities in the T and N regions. (C) The percentages of S100A9⁺CD34⁺ cells among the total CD34⁺ cells in the N and T regions. (F) The percentages of S100A9⁺CD56⁺ cells among the total CD56⁺ cells in the N and T regions. (I) The percentages of S100A9⁺CD20⁺ cells among the total CD20⁺ cells in the N and T regions. (L) The percentages of S100A9⁺CD3⁺ cells among total CD3⁺ cells in the N and T regions (n = 4 - 7). Scale bar = 50 μm. The results are the means ± SEM (bars); NS, no significance; ND, not detected.