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A representative mixed lymphocyte reaction of indirect antigen presentation. B10 DC was pulsed with C3H splenocyte lysate overnight and used as stimulators to activate B10 enriched T-cells. The result showed that the proliferating capacities of T-cells from aged were lower than those of T-cells from young mice (0.328 ± 0.004 O.D. versus 0.491 ± 0.008 O.D. at responders/stimulatory cells = 100/1, p = 0.001).
