Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 7;13(12):16009–16023. doi: 10.18632/aging.203093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2021 Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Calycosin treatment reduces BATF levels in breast cancer cells. (A) RT-qPCR analysis shows relative levels of BATF mRNA in T47D and MCF-7 cells treated with different concentrations of calycosin. (B) Western blot analysis shows the relative levels of BATF protein in T47D and MCF-7 cells treated with different concentrations of calycosin. (C, D) RT-qPCR and western blot analyses show the relative levels of BATF mRNA and protein in control, BATF-overexpressing, and BATF-silenced T47D and MCF-7 cells. (E) RT-qPCR analysis shows relative levels of CD147, MMP-2, and MMP-9 transcripts in control, BATF-overexpressing, and BATF-silenced T47D and MCF-7 cells. (F) Western blot analysis shows relative levels of CD147, MMP-2, and MMP-9 proteins in control, BATF-overexpressing, and BATF-silenced T47D and MCF-7 cells. (G) Transwell invasion assay results show invasiveness of control, BATF-overexpressing, and BATF-knockdown T47D and MCF-7 cells. (H) Wound healing assay results show migration rates of control, BATF-overexpressing, and BATF-knockdown T47D and MCF-7 cells. The data were presented as means ± SD. *P<0.05; **P<0.01; ***P<0.001.