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. 2021 Jun 7;13(12):16009–16023. doi: 10.18632/aging.203093

Figure 3.

Figure 3

Calycosin counteracts pro-metastatic effects of BATF in breast cancer cells. (A) RT-qPCR analysis shows relative levels of CD147, MMP-2, and MMP-9 transcripts in control and calycosin-treated BATF-overexpressing T47D and MCF-7 cells. (B) Western blot analysis shows relative levels of CD147, MMP-2, and MMP-9 proteins in control and calycosin-treated BATF-overexpressing T47D and MCF-7 cells. (C) Representative images show the migration ability of control and calycosin-treated BATF-overexpressing T47D and MCF-7 cells at 0 and 24 h. (D) Transwell invasion assay results show invasiveness of control and calycosin-treated BATF-overexpressing T47D and MCF-7 cells. The data were represented as means ± SD. *P<0.05; **P<0.01; ***P<0.001.