Table 1. A comparison between analyzed parameters in cells lacking active DNMT2/TRDMT1 gene (D-NIC cells) and in cells with functional DNMT2/TRDMT1 gene (C-NIC cells) during doxorubicin- or etoposide-induced senescence (35 nM DOX or 1 μM ETOPO).
| Parameter | C-NIC cells versus D-NIC cells | |||||||
| HeLa | MDA-MB-231 | U-2 OS | U-251 MG | |||||
| DOX | ETOPO | DOX | ETOPO | DOX | ETOPO | DOX | ETOPO | |
| cell cycle | - | G0/G1↑ | G2/M↑ | G2/M↑ | - | - | G2/M↑ | G2/M↑ |
| SA-β-gal activity | - | - | - | - | - | - | ↓ | ↓ |
| p21 | - | - | ↓ | ↓ | - | - | ↓ | ↓ |
| apoptosis | ↑ | - | ↑ | ↑ | ↓ | ↑ | ↓ | - |
| senolytic activity of 1 μM AZA |
↑ (apoptosis) |
↑ (apoptosis) |
- | - | - |
↑ (necrosis) |
- | - |
| ROS | ↑ | - | - | ↑ | ↓ | ↓ | ↑ | ↑ |
| DNA damage | - | ↓ | ↑ | - | - | ↓ | ↑ | - |
| micronuclei | - | ↓ | - | - | - | - | ↓ | ↓ |
| pATM(+)pH2AX(+) | ↓ | ↓ | - | ↓ | ↑ | ↑ | ↓ | - |
| 53BP1 | ↑ | ↑ | ↑ | - | - | ↓ | ↓ | - |
| RNA-DNA hybrids | - | - | - | - | - | - | ↓ | ↓ |
| RAD51 | ↑ | - | - | - | - | - | ↓ | ↓ |
| RAD52 | - | - | - | - | - | ↓ | ↓ | ↓ |
| XRCC1 | ↑ | - | - | ↓ | ↓ | ↓ | ↑ | ↑ |
| LC3 | - | ↓ | - | ↓ | - | - | ↓ | ↓ |
| p62 | ↑ | ↑ | - | ↓ | - | ↑ | - | - |
| lysosomes | ↑ | ↑ | - | - | ↑ | ↑ | ↓ | - |
| NF-κB | ↑ | - | - | - | - | ↓ | - | - |
| IL-1β | - | - | - | - | - | ↑ | ↑ | ↑ |
| IL-6 | - | ↑ | - | - | - | - | - | - |
| IL-8 | - | - | - | ↓ | - | - | ↓ | ↓ |
| NSUN1 | ↓ | - | ↓ | - | - | - | - | ↑ |
| NSUN2 | - | - | ↑ | ↑ | ↓ | ↓ | - | ↓ |
| NSUN3 | - | - | ↓ | - | - | ↑ | - | ↓ |
| NSUN4 | - | - | ↓ | - | - | - | - | - |
| NSUN5 | - | - | - | - | - | - | - | ↓ |
| NSUN6 | - | - | - | - | - | - | ↑ | ↑ |
| cytoplasmic 5-mC | ↑ | ↓ | - | - | ↓ | - | ↓ | ↓ |
| nuclear 5-mC | - | - | - | ↑ | ↓ | - | - | ↓ |
Four different cancer cellular models were considered, namely HeLa, MDA-MB-231, U-2 OS and U-251 MG cells. ↑ (red), statistically significant increase in analyzed parameter compared to corresponding C-NIC cells; ↓ (green), statistically significant decrease in analyzed parameter compared to corresponding C-NIC cells; - (gray), no statistically significant changes compared to corresponding C-NIC cells.