Table 1. Primer sequences for RT-qPCR.
Genes | Primer sequences |
IL-6 | F: 5′-AAATTCGGTACATCCTCGACGG-3′ |
R: 5′-GGAAGGTTCAGGTTGTTTTCTGC-3′ | |
STAT3 | F: 5′-GTCAGAT -GCCAAATGCG-3′ |
R: 5′-TGATGTACCCT -TCGTTCT-3′ | |
JAK2 | F: 5′-GGGTGTTCGCGTCGCCACTT-3′ |
R: 5′-CAGATCGGGCGAC-CAGAGCGC-3′ | |
CyclinD1 | F: 5′-GCTGCGAAGTGGAAACCATC-3′ |
R: 5′-CCTCCTTCTGCACACATTTGAA-3′ | |
GAPDH | F: 5′-ACCACAGTCCATGCCATCAC-3′ |
R: 5′-TCCACCACCCTGTTGCTGTA-3′ |
Abbreviations: RT-qPCR: reverse transcription quantitative polymerase chain reaction; IL-6: interleukin-6; STAT3: Signal transducers and activators of transcription 3; JAK2: Janus kinase 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.