Figure 10.

Inhibitory effects of AS on AKT, mTOR, and NFκB signaling pathways in SW620 cells. (A) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of p(Ser473)-AKT and AKT. (B) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of p(Ser2448)-mTOR and mTOR. (C) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of nuclear and cytosolic NFκB (p65). (D) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of nuclear and cytosolic β-catenin. Relative changes in the intensities of protein bands were measured using commercially available quantitative software with the control representing as 1-fold.