Table 4. Phenotypic and genotypic identification of methicillin-resistant Staphylococcus (MRS) isolated from samples of human milk in a Teaching Hospital from Sao Paulo State, Brazil.
AST Cefoxitin | AST Oxacillin | AST Penicillin | MIC Oxacillin | PCR mecA | |
---|---|---|---|---|---|
n/T (%) | n/T (%) | n/T (%) | n/T (%) | n/T (%) | |
CPS | |||||
S. aureus | 01/15 (6.67) | 02/15 (13.33) | 05/15 (33.33) | 0/15 (0) | 02/15 (13.33) |
Staphylococcus spp. | 02/15 (13.33) | 03/15 (20) | 09/15 (60) | 1/15 (6.67) | 06/15 (40) |
Total | 03/15 (20) | 05/15 (33.33) | 14/15 (93.33) | 1/15 (6.67) | 08/15 (53.33) |
CoNS | |||||
S. epidermidis | 13/40 (32.50) | 16/40 (40) | 25/40 (62.50) | 12/40 (30) | 22/40 (55) |
S. lugdunensis | 0/40 (0) | 0/40 (0) | 01/40 (2.50) | 01/40 (2.50) | 0/40 (0) |
S. saprophyticus | 03/40 (7.50) | 04/40 (10) | 05/40 (12.50) | 02/40 (5) | 03/40 (7.50) |
S. simulans | 01/40 (2.50) | 01/40 (2.50) | 02/40 (5) | 01/40 (2.50) | 02/40 (5) |
S. xylosus | 01/40 (2.50) | 01/40 (2.50) | 02/40 (5) | 02/40 (5) | 01/40 (2.50) |
Staphylococcus spp. | 03/40 (7.50) | 02/40 (5) | 03/40 (7.50) | 0/40 (0) | 02/40 (5) |
Total | 21/40 (52.50) | 24/40 (60) | 38/40 (95) | 18/40 (45) | 30/40 (75) |
AST = antimicrobial susceptibility testing by the disk diffusion method; MIC = minimal inhibitory concentration; PCR = polymerase chain reaction; mecA = gene encoding methicillin resistance; n = number of isolates; T = total of isolates; CPS = Coagulase-positive staphylococci; CoNS = Coagulase-negative staphylococci.