Figure 1.
Crystal structure and flexibility of the zRET CLD(1-4) module
(A) Schematic of zebrafish RET receptor tyrosine kinase. CLD, cadherin-like domains; CRD, cysteine-rich domain; TM, transmembrane helix; JM, juxtamembrane domain; KD, kinase domain.
(B) Orthogonal views of zRETCLD1-4 colored as in (A). The calcium-binding site between CLD(2-3) has three calcium ions as green spheres with coordinating ligands as sticks and waters as red spheres.
(C) Close-up view of the coordination shell for the three calcium atoms between CLD2 and CLD3.
(D) Close-up of the interface between CLD3 and CLD4 centered on R272, selected side chains shown as sticks and dashed lines for hydrogen bonds.
(E) Superposition of chains A and B within the crystallographic asymmetric unit.