Table 2.
EM data acquisition and processing statistics
| zRGα1a C2 map | zRGα1a symmetry-expanded map | zR15AL negative stain map | |
|---|---|---|---|
| EMDB: | EMD-11822 | EMD-11822 | EMD-11777 |
| PDB: | 7AML | ||
| Magnification | 46,296 | 46,296 | 40,719 |
| Voltage (kV) | 300 | 300 | 120 |
| Electron exposure (e−/Å2) | 48.6 | 48.6 | – |
| Defocus range (μm) | 1.4–3.5 | 1.4–3.5 | 1.0–1.5 |
| Pixel size (Å) | 1.08 | 1.08 | 3.44 |
| Symmetry imposed | C2 | C1 | C2 |
| Initial particle images | 2,424,600 (dataset 1), 1,393,023 (dataset 2) | – | 27,551 |
| Final particle images | 382,547 (360,189 dataset 1 and 22,358 dataset 2) | 765,094 | 6,519 |
| Map resolution (Å) | 3.3 | 3.5 | 26 |
| FSC threshold | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 12–3.3 | 11–3.5 |
| Refinement | |
| Initial model, PDB: | 7AMK, 7AB8 |
| Model resolution (Å) | 4.2 |
| FSC threshold | 0.5 |
| Map sharpening B factor (Å2) | −75 |
| Non-hydrogen atoms | 16,020 |
| Protein residues | 1,996 |
| Ligands | 8 |
| N-Glycans | 16 |
| Protein | 122.4 |
| Ligands | 111.6 |
| Bond lengths (Å) | 0.004 (0) |
| Bond angles (°) | 0.646 (6) |
| Validation | |
| MolProbity score | 1.85 |
| Clashscore | 9.45 |
| Poor rotamer (%) | 0.89 |
| Favored | 94.94 |
| Allowed | 5.06 |
| Disallowed | 0.0 |
RMSD, root-mean-square deviation.