Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Aquifex aeolicus) | Aq_880 | GenBank | AAC07003.1 | |
| Gene (Halorhodospira halophila) | Hhal_2243 | GenBank | ABM63007.1 | |
| Strain, strain background (Escherichia coli) | Rosetta (DE3) | Merck Novagen | 70954–3 | |
| Strain, strain background (Escherichia coli) | Lemo21 (DE3) | NEB Biolabs | HC2528J | |
| Antibody | 6×His Tag HRP (mouse monoclonal) | Thermo Fisher Scientific, Invitrogen | Catalog #MA1-21315-HRP | Dilution 1:5000 |
| Commercial assay or kit | Gel Filtration Markers Kit for Protein Molecular Weights12–200 kDa | Merck Sigma-Aldrich | MWGF200 | |
| Chemical compound, drug | Apoferritin | Merck Sigma-Aldrich | A3660 | |
| Software algorithm | Adobe Illustrator CC | Adobe | Version 25.0 | |
| Software algorithm | Adobe Photoshop CC | Adobe | Version 22.0.0 | |
| Software algorithm | CryoSparc | doi: 10.1038/nmeth.4169 | v3.1 | |
| Software algorithm | CTFfind4 | doi: 10.1016/j.jsb.2015.08.008 | Version 4.1.14 | |
| Software algorithm | Topaz | doi: 10.1038/s41592-019-0575-8 | ||
| Software algorithm | COOT | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | Version 0.9.3 | |
| Software algorithm | Phenix | http://www.phenix-online.org | Version 1.18 | |
| Software algorithm | UCSF ChimeraX | https://www.cgl.ucsf.edu/chimerax/ | Version 1.2.5 | |
| Software algorithm | Molprobity | Duke Biochemistry,http://molprobity.biochem.duke.edu/ | ||
| Software algorithm | PyMol 2 | https://pymol.org/2/ | Version 2.0.6 | |
| Software algorithm | AcquireMP | Refeyn (https://www.refeyn.com) | v.2.3 |