Table 1. Processing activities of Aq880 variants.
n.d., no cleavage detectable.
| Aq880 variant | kobs (min-1) (±SD) |
Substrate cleaved after 25 min (in %) |
|---|---|---|
| wt 50 nM wt 500 nM | 0.62 ± 0.15 2.06 ± 0.42 |
95 98 |
| ∆184–191 50 nM ∆184–191 500 nM |
0.22 ± 0.08 1.41 ± 0.07 |
46 97 |
| ∆181–191 500 nM ∆179–191 500 nM |
(4 ± 1) × 10–3
(7 ± 3) × 10–3 |
9 15 |
| ∆177–191 500 nM | – | n.d. |
| R125A 50 nM R125A 500 nM |
(15 ± 3) × 10−4
(16.1 ± 0.3) × 10–2 |
2 26 |
| R129A 500 nM | – | n.d. |
| R125A/R129A 500 nM | – | n.d. |
| K119A/R123A/R125A/K127A/R129A 500 nM | – | n.d. |